The first commercial flight out of Dubai since the war began, carrying more than 200 Australians on board, landed in Sydney late Wednesday night.
The arrivals were greeted at the airport with hugs and tears from family members who had been anxiously awaiting their return.
Foreign Minister Penny Wong said three more flights were scheduled to leave the UAE on Thursday.
"We are hoping, all of Australia is hoping, that those flights can depart safely," she told Nine's Today program.
There are 24,000 Australians in the UAE, with about 115,000 across the Middle East.
Six crisis response teams are on their way to the region to provide extra consular support in response to "unprecedented" demand.
Landing on Australian soil, Iman Krayem was surrounded by her son Youssef and husband Nazih.
She was stuck in the United Arab Emirates for several nights, on her way to see her sick father in Lebanon, when Iranian missiles struck the gilded city in response to a barrage of US and Israeli attacks.
"I was crying non-stop," she told AAP minutes after clearing customs.
"It was so stressful, I didn't have my luggage, I had no clothes but I am happy to be back home now."
Among the more than 200 passengers who arrived were a group of high school students travelling to Istanbul for a robotics competition.
Charity worker Hawra Khalil, another arrival on Wednesday, was in Lebanon on a humanitarian trip feeding war-torn children in several cities.
Heeding the Australian government's travel warning, Ms Khalil managed to catch a flight to Dubai where she was grounded with a colleague.
She said being caught in a conflict zone in the relative comfort of the Gulf monarchy, where she felt buildings shake for a few days, drove home how other citizens in Arab countries have been faring in recent years.
"I just got a glimpse of it in Dubai and I had seen what people in Lebanon go through on a daily basis ten-fold," she told AAP.
"It is scary, you feel threatened but I have it so much easier because what I witnessed is innocent families and innocent children starving and in poverty."
Iran has fired hundreds of drones in recent days across several countries including Kuwait, Qatar and Saudi Arabia, targeting American diplomatic and military sites in retaliation for US and Israeli strikes.
Mining executive Troy Barker had arrived in Dubai for just a day and was at a popular horse race on Saturday, where the Emirati ruler was also in attendance, when he saw drones and missiles across the city's skyline.
"I saw a couple of missiles but I've worked in Africa for 20 years so I've seen many things," he said.
He praised communications from Emirates airlines and the authorities on the ground, saying he was lucky to be home.
Senator Wong has previously revealed Prime Minister Anthony Albanese had lobbied Emirati president Mohammed bin Zayed Al Nahyan to ensure their safe exit.
