A tractor towing agricultural equipment and a 4WD Land Rover have collided on Winter Rd, Nalleenna.
The incident occurred around 4pm, about 300 metres from the Freeman Rd intersection.
Passengers of the 4WD Land Cruiser - a 14-year-old girl and her grandfather in his 60s- were taken to hospital by ambulance.
The tractor driver did not sustain any injuries.
The Rochester SES unit attended to free the occupants of the 4WD Land Cruiser.
Campaspe Highway Patrol Leading Senior Constable Paul McCracken is currently investigating the accident.
Anyone who may have witnessed the accident is asked to contact police via Crime Stoppers on 1800 333000.